Patch biogeography under intermittent barriers: macroevolutionary consequences of microevolutionary processes.

The processes that generate biodiversity start on a microevolutionary scale, where each individual's history can impact the species' history. This manuscript presents a theoretical study that examines the macroevolutionary patterns that emerge from the microevolutionary dynamics of populations inhabiting two patches. The model is neutral, meaning that neither survival nor reproduction depends on a fixed genotype, yet individuals must have minimal genetic similarity to reproduce. We used historical sea level oscillation over the past 800 thousand years to hypothesize periods when individuals could migrate from one patch to another. In our study, we keep track of each speciation and extinction event, build the complete and extant phylogenies, and characterize the macroevolutionary patterns regarding phylogeny balance, acceleration of speciation, and crown age. We also evaluate ecological patterns: richness, beta diversity, and species distribution symmetry. The balance of the complete phylogeny can be a sign of the speciation mode, contrasting speciation induced by migration and isolation (vicariance). The acceleration of the speciation process is also affected by the geographical barriers and the duration of the isolation period, with high isolation times leading to accelerated speciation. We report the correlation between ecological and macroevolutionary patterns and show it decreases with the time spent in isolation. We discuss, in light of our results, the challenge of integrating present-time community ecology with macroevolutionary patterns.

Development of nanobiosilicate, tricalcium phosphate and chlorhexidine materials for biomineralization with crystallographic similarity to hydroxyapatite and biomodified collagen.

OBJECTIVES: The aim of this work is to test experimental cements, doped with a silicate based bioactive nanoparticle (NanoBiosilicate). Methods, we synthesized a glass nanoparticle by Sol-Gel Stöber method, used to be incorporated in a dental material for endodontic uses. MATERIALS AND METHODS: We assess the mineralizing properties and biocompatibility. Besides the crystallography characterization of the resultant new crystals. Results, After analysis, and comparison with commercial materials, the material tested was similar in mechanical properties required by ISO, The ion release was effective after 2 hr. of setting and the novel material was cell compatible accepted by ISO. RESULTS: We found new formed Calcium Phosphate peaks in the spectroscopic analysis (FTIR), remarkably the crystals formed were comparable to hydroxyapatite when analyzed with a Selected Area Electron Diffractometer, with rings of 2.84 Å for 002, and the 2.77 Å is also visible for 210. The 6.83 Å and 6.88 Å, for respective 222 and 004. The incorporation of Chlorhexidine was not detrimental for this property, Significance, the features mentioned represented a progress in biomineralization field that was associated to an improved mineral structure formation with increased crystallographic similarity to natural hydroxyapatite. When chlorhexidine was added a favorable biomodification of the remaining collagen in dentinal walls and antimicrobial activity potential were also observed.

Evolutionary rescue on genotypic fitness landscapes.

Populations facing adverse environments, novel pathogens or invasive competitors may be destined to extinction if they are unable to adapt rapidly. Quantitative predictions of the probability of survival through adaptation, evolutionary rescue, have been previously developed for one of the most natural and well-studied mappings from an organism's traits to its fitness, Fisher's geometric model (FGM). While FGM assumes that all possible trait values are accessible via mutation, in many applications only a finite set of rescue mutations will be available, such as mutations conferring resistance to a parasite, predator or toxin. We predict the probability of evolutionary rescue, via de novo mutation, when this underlying genetic structure is included. We find that rescue probability is always reduced when its genetic basis is taken into account. Unlike other known features of the genotypic FGM, however, the probability of rescue increases monotonically with the number of available mutations and approaches the behaviour of the classical FGM as the number of available mutations approaches infinity.

Increased dietary Trp, Thr, and Met supplementation improves growth performance and protein deposition of salmonella-challenged growing pigs under poor housing conditions.

Highly intensified rearing conditions and precarious sanitary management predispose pigs to immune system activation, altered amino acid (AA) metabolism, and decreased growth performance. Thus, the main objective of this study was to evaluate the effects of increased dietary tryptophan (Trp), threonine (Thr), and methionine + cysteine (Met + Cys) supplementation on performance, body composition, metabolism, and immune responses of group-housed growing pigs under challenging sanitary conditions. A hundred and twenty pigs (25.4 ± 3.7 kg) were randomly assigned to a 2 × 2 factorial arrangement, consisting of two sanitary conditions (SC, good [GOOD] or salmonella-challenge and poor housing condition [Salmonella Typhimurium (ST) + POOR]) and two diets, control (CN) or supplemented with AA (Trp, Thr, and Met + Cys:Lys ratios 20% higher than those of the CN diet [AA>+]). Pigs were followed during the growing phase (25-50 kg) and the trial lasted 28 d. The ST + POOR SC pigs were challenged with Salmonella Typhimurium and raised in a poor housing condition. The ST + POOR SC increased rectal temperature, fecal score, serum haptoglobin, and urea concentration (P < 0.05) and decreased serum albumin concentration (P < 0.05) compared with GOOD SC. Body weight, average daily feed intake, average daily gain (ADG), feed efficiency (G:F), and protein deposition (PD) were greater in GOOD SC than in ST + POOR SC (P < 0.01). However, pigs housed in ST + POOR SC fed with AA+ diet had lower body temperature (P < 0.05), increased ADG (P < 0.05) and nitrogen efficiency (P < 0.05), and a tendency for improved PD and G:F (P < 0.10) compared with CN diet fed pigs. Regardless of the SC, pigs fed AA+ diet had lower serum albumin (P < 0.05) and tended to decrease serum urea levels (P < 0.10) compared with CN diet. The results of this study suggest that the ratio of Trp, Thr, and Met + Cys to Lys for pigs are modified by sanitary conditions. Furthermore, supplementation of diets with a blend of Trp, Thr, and Met + Cys improves performance, especially under salmonella-challenge and poor housing conditions. Dietary tryptophan, threonine, and methionine supplementation can modulate immune status and influence resilience to sanitary challenges.

Immune system activation alters pigs' physiology and metabolism, increasing maintenance requirements and reducing voluntary feed intake and weight gain. Dietary functional amino acid supplementation (tryptophan, threonine, and methionine) is a strategy to support the immune system activation for immune components production, maintenance of the gut barrier integrity, and reduction of the oxidative status. Additionally, amino acid supplementation may mitigate growth performance losses. In this context, this study was conducted to investigate the effect of diets with or without tryptophan, threonine, and methionine supplementation on the performance and immune system activation of growing pigs under a sanitary challenge. The amino acid supplementation mitigated the immune system activation of challenged growing pigs and improved growth performance when compared to pigs fed diets with no supplementation. The functional amino acid supplementation may be an efficient nutritional strategy to optimize health and growth performance of immune-challenged pigs.

Agenesia of salivary glands: incidental findings in multidetector computerized tomography.

Agenesis of the salivary glands is an extremely uncommon finding, and in the majority of cases, it is associated with facial syndromes or malformations. Reports in the literature have, however, indicated that agenesis of the major salivary glands can occur in isolation, and this anomaly is believed to occur due to a failure in the developmental process. Herein, we present two cases of isolated unilateral agenesis of major salivary glands.

Phenotypic evolution as an Ornstein-Uhlenbeck process: The effect of environmental variation and phenotypic plasticity.

Here we investigate phenotypic evolution from the perspective of the Ornstein-Uhlenbeck (OU) process. Evolutionarily speaking, the model assumes the existence of stabilizing selection toward a phenotypic optimum. The standard (OU) model is modified to include environmental variation by taking a moving phenotypic optimum and endowing organisms with phenotypic plasticity. These two processes lead to an effective fitness landscape, which deforms the original. We observe that the simultaneous occurrence of environmental variation and phenotypic plasticity leads to skewed phenotypic distributions. The skewness of the resulting phenotypic distributions strongly depends on the rate of environmental variation and strength of selection. When generalized to more than one trait, the phenotypic distributions are not only affected by the magnitude of the rate of environmental variation but also by its direction. A remarkable feature of our predictions is the existence of an upper bound for the critical rate of environmental variation to allow population persistence, even if there is no cost associated with phenotypic plasticity.

A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Doses.

Salmonella Typhimurium is the most frequent serovar in pigs and causes infections in humans. However, the dosage used for experimentation is not well defined. The present study aimed to evaluate a dosage for oral inoculation with Salmonella Typhimurium to assess immunological and growth performance alterations in pigs. Gilts were randomly allocated into one of three experimental treatments: no Salmonella Typhimurium inoculation (Basal), or oral inoculation of 1 × 108 or 1.5 × 108 colony-forming units of Salmonella Typhimurium. Growth rate, rectal temperature, and fecal Salmonella shedding were recorded. Blood samples were taken. Inoculated pigs shed the bacteria for up to 7 days, but no differences were observed between the groups. No differences were observed in rectal temperature, body weight, or average daily feed intake. However, reductions in average daily gain (-17 and -22%) and feed efficiency (-14 and -20%) were observed in pigs inoculated with 1 × 108 and 1.5 × 108 colony-forming units, respectively. The hemoglobin and hematocrit concentrations increased in challenged pigs compared to Basal pigs. The oral dosage of 1.5 × 108 colony-forming units of Salmonella Typhimurium is suitable for activating the immune system of pigs and assessing the impact of Salmonella on pig performance.

A blend of functional amino acids and grape polyphenols improves the pig capacity to cope with an inflammatory challenge caused by poor hygiene of housing conditions.

BACKGROUND: Dietary supplementation with a blend of functional amino acids (AA) and grape extract polyphenols contributes to preserve intestinal health and growth performance of piglets during the post-weaning period. In the present experiment, we assessed if a supplementation with a mix of AA and grape extract polyphenols during the post-weaning period would persist to improve the pig capacity to cope with a subsequent challenge caused by poor hygiene of housing conditions. Eighty pigs weaned at 28 days of age were fed a standard diet supplemented (AAP) or not (CNT) with 0.2% of a blend of AA (glutamine, arginine, cystine, valine, isoleucine, and leucine) and grape extract polyphenols during the post-weaning period (from week 0 to 6). At week 6, pigs were transferred to a growing unit where 50% of pigs previously fed AAP and CNT diets were housed in good and the other 50% in poor hygiene conditions for 3 weeks (from week 7 to 9; challenge period). All pigs were fed a standard growing diet that was not supplemented with AAP. We measured pig growth performance, plasma indicators of inflammation, digestive integrity, and oxidative status, and scored fecal consistency. Differences were considered significant at P ≤ 0.05. RESULTS: One week post-weaning, pigs fed AAP had lower plasma concentrations of haptoglobin than CNT pigs (P = 0.03). Six weeks post-weaning, plasma concentrations of diamine oxidase (DAO) were lower (P = 0.03) whereas those of vitamin E and A were greater (P ≤ 0.05) in pigs fed AAP compared to CNT pigs. The prevalence of diarrhea was higher in CNT pigs compared to AAP pigs (P < 0.01). During the challenge period, only pigs previously fed CNT diet had lower growth rate in poor than good conditions (P ≤ 0.05). They had also greater plasma concentrations of haptoglobin and oxidative stress index (OSI) and lower plasma concentrations of vitamin E in poor than good hygiene conditions (P ≤ 0.05). CONCLUSIONS: Pigs fed AAP diet during post-weaning had less diarrhea and plasma concentrations of a digestive integrity marker, as well as greater plasma concentrations of antioxidant indicators during the post-weaning period. The beneficial effects of AAP supplementation persisted after the post-weaning period as evidenced by the absence of effects of the hygiene challenge on growth and health indicators in pigs previously fed APP. This clearly indicated a greater ability of pigs fed AAP to cope with the poor hygiene conditions.

Effects of lowering dietary protein content without or with increased protein-bound and feed-grade amino acids supply on growth performance, body composition, metabolism, and acute-phase protein of finishing pigs under daily cyclic heat stress.

This study investigated the effects of a low-protein diet with or without an increase in dietary protein and feed-grade amino acids (AAs) on the growth performance, body composition, metabolism, and serum acute-phase proteins of finishing pigs reared in thermoneutrality or cyclic heat stress conditions. A total of 90 gilts (67.7 ± 6.2 kg) were distributed in a 2 × 3 factorial arrangement (two ambient temperatures and three diets). Ambient temperatures (AT) were thermoneutral (TN, 22 °C for 24 h) and cyclic heat stress (CHS, 12 h to 35 °C and 12 h to 22 °C). The evaluated diets (D) were high crude protein (HP); low CP-free AA-supplemented diets (LPAAs); low CP-free AA-supplemented diets and digestible Lys level (+20%), and Lys:AA ratios above recommendations (LPAA+). The experimental period lasted 48 d (two experimental phases: days 0-27 and days 28-48, respectively). CHS pigs had higher skin temperature (P < 0.05) than TN pigs. Pigs in CHS had higher rectal temperature (P < 0.05) than TN pigs until day 38 but similar (P > 0.10) to TN pigs from 38 to 45 d. For the entire experiment, CHS pigs had lower (P < 0.05) final BW, average daily gain and daily feed intake, net energy intake, body lipid, bone mineral, lipid deposition, energy retention, Lys and CP intake, and nitrogen excretion than TN pigs. The level of CP intake impacted nitrogen excretion, nitrogen retention efficiency, and urea as pigs fed HP had the highest values, and pigs fed LPAA had the lowest values (P < 0.05). On day 27, CHS pigs had lower (P < 0.05) free triiodothyronine than TN pigs. LPAA+ pigs had lower (P < 0.05) insulin than LPAA. On day 48, CHS pigs had lower (P < 0.05) thyroxine, albumin, and lactate than TN pigs. On day 27, pigs fed LPAA+ had higher (P < 0.05) lactate than pigs fed HP or LPAA. Both AT and D were enough to stimulate the immune system as CHS pigs had lower (P < 0.05) transferrin and 23-kDa protein levels than TN pigs, and HP pigs had higher haptoglobin than LPAA on day 27. These results confirm the deleterious effects of high AT on performance, body composition, metabolism, and immune system stimulation in finishing pigs. These data also show that a diet with low levels of CP can be provided to pigs in CHS without affecting performance and body composition while reducing nitrogen excretion. However, the use of a diet with an AA level above the requirements obtained by increasing intact protein and free AA did not attenuate the impact of CHS on performance and body composition of pigs.

High ambient temperature and air humidity are the most important climatic factors that jeopardize pig production. Multiple strategies can be applied for pigs under heat stress, including recent research to improve understanding the use of nutrition to attenuate the impact of heat stress. Heat stress impairs digestion, absorption, and amino acid metabolism with changes in amino acid requirements. Updates on the nutritional assessment strategies by differing the diets by protein and amino acid content (protein-bound or feed-grade) seem to be efficient tools for pork producers as amino acids play a functional role in challenged pigs apart from the beneficial effects on performance.

Intrauterine growth restriction and its impact on intestinal morphophysiology throughout postnatal development in pigs.

Intrauterine growth restriction (IUGR) compromises fetal development, leading to low birth weight, and predisposes to gastrointestinal disorders. Pigs that suffered IUGR present poor postnatal development, resulting in great economic losses to the industry. The small intestine may be involved with impaired development, but studies investigating this issue are still limited. Thus, the present study aimed to investigate small intestine morphofunctional alterations in IUGR pigs throughout the production phases (birth to 150 days). IUGR pigs presented lower body weight from birth to the finishing phase (P < 0.05). Although histomorphometrical parameters were not affected during the pre-weaning period, their commitment was observed specifically in the duodenum of the IUGR group at older ages (P < 0.05). The most detrimental effects on the small intestine, such as deeper duodenum crypts' depth, lower villus height:crypt depth ratio and absorptive area, increased apoptosis and lower proliferation of the duodenum epithelium were noticed at 70 days of age (P < 0.05). Additionally, IUGR pigs presented the lowest chymotrypsin and amylase activities at 70 and 150 days of age, respectively (P < 0.05). These findings may contribute to the elucidation of morphofunctional disorders of the small intestine in IUGR pigs throughout the different production phases, suggesting that poor postnatal development may be due to intestinal damage.

Genetic selection modulates feeding behavior of group-housed pigs exposed to daily cyclic high ambient temperatures.

This study was conducted to evaluate the effect of genetic selection (Lines A and B; Line A pigs have a greater proportion of Pietrain genes than those from Line B and therefore, selected for improved lean tissue accretion) on the feeding behavior of group-housed pigs exposed to daily cyclic high ambient temperatures. Feeding behavior of 78 barrows housed together in a single room was recorded in real time by five automatic feeders. The feeders registered each visit of each pig (day, hour, min, and second) and the amount of feed requested. Daily cyclic high ambient temperature was induced exposing pigs at 22°C from 18.00 to 10.00 h and 30°C from 10.01 to 17.59 h. From this temperature variation, day-period was divided into: 22°C(06-10h), from 6.00 to 10.00 h; 30°C(10-18h), from 10.01 to 17.59 h; and 22°C(18-06h), from 18.00 to 5.59 h. Meal criteria was estimated based on the probability of animals starting a new feeding event within the next minute since the last visit (Pstart). After defining the meal criteria, the number of meals (n), feed intake rate (g/min), feed intake (g/meal), feeder occupancy (min/meal), and interval between meals (min) of each animal were calculated. Greatest probability of starting to feed was observed at 22°C(06-10h), followed by 30°C(10-18h) and then 22°C(18-06h). Regardless of time period, pigs from line A had greater feed intake rate and lower feed intake, feed occupancy per meal and probability of starting a meal when compared with line B pigs. Only line A pigs had greater feed intake and feeder occupancy per meal at 22°C(18-06h) than remainder of the day. This indicates that pig feeding pattern is strongly related to the circadian rhythm. However, the genetic selection for improved lean tissue accretion may modulate pigs feeding behavior under daily cyclic high ambient temperatures.

Rate of environmental variation impacts the predictability in evolution.

In the two last decades, we have improved our understanding of the adaptive evolution of natural populations under constant and stable environments. For instance, experimental methods from evolutionary biology have allowed us to explore the structure of fitness landscapes and survey how the landscape properties can constrain the adaptation process. However, understanding how environmental changes can affect adaptation remains challenging. Very little progress has been made with respect to time-varying fitness landscapes. Using the adaptive-walk approximation, we survey the evolutionary process of populations under a scenario of environmental variation. In particular, we investigate how the rate of environmental variation influences the predictability in evolution. We observe that the rate of environmental variation not only changes the duration of adaptive walks towards fitness peaks of the fitness landscape, but also affects the degree of repeatability of both outcomes and evolutionary paths. In general, slower environmental variation increases the predictability in evolution. The accessibility of endpoints is greatly influenced by the ecological dynamics. The dependence of these quantities on the genome size and number of traits is also addressed. To our knowledge, this contribution is the first to use the predictive approach to quantify and understand the impact of the speed of environmental variation on the degree of parallelism of the evolutionary process.

Effects of sequential feeding with adjustments to dietary amino acid concentration according to the circadian rhythm on the performance, body composition, and nutrient balance of growing-finishing pigs.

This study aimed to evaluate the effects of a sequential feeding program (SEQ) with diets varying in amino acid (AA) concentrations in the first and last 12 h of the day on the performance, body composition, and nutrient balance of growing-finishing pigs. Sixty-eight castrated male pigs were distributed in four treatments: a daily feeding program (DP) and three SEQs. In the DP, dietary requirements of AA were adjusted daily. In the SEQ, dietary daily requirements of AA were adjusted every 12 h, providing a low AA concentration in period 1 (P1; 00:00-11:59 h) and a high AA concentration in period 2 (P2; 12:00-23:59 h). In the SEQ, three different levels of low and high AA concentrations were evaluated: ±20%, ±30%, and ±40%. The experiment lasted 82 days and was divided into phase 1 (25-50 kg body weight; BW), phase 2 (50-70 kg BW), and phase 3 (70-100 kg BW). During phase 1, irrespective of dietary AA concentration, SEQ pigs had higher lysine intake, protein gain, and phosphorus efficiency than DP pigs (P ≤ 0.05). Pigs in the SEQ showed a tendency for greater average daily gain, body protein, and body lipids compared to the DP pigs (P ≤ 0.10). During phase 2, SEQ pigs showed a tendency for higher average feed intake in P2 compared to DP pigs (P = 0.07); consequently, average daily gain, body protein, and phosphorus retention tended to increase (P ≤ 0.10). During phase 3, SEQ pigs had a higher average feed intake in P2 than DP pigs (P = 0.03). However, they had a similar body composition (P > 0.05) and a tendency for higher nitrogen excretion (P = 0.06) than DP pigs. Our results suggest that SEQ is an effective approach for improving the performance and body composition of growing pigs.

Assessment of digestible lysine requirements in lipopolysaccharide-challenged pigs.

To evaluate the effect of an Escherichia coli lipopolysaccharide (LPS) challenge on the digestible lysine (Lys) requirement for growing pigs, a nitrogen (N) balance assay was performed. Seventy-two castrated male pigs (19 ± 1.49 kg body weight [BW]) were allocated in a 2 × 6 factorial design composed of two immune activation states (control and LPS-challenged) and six dietary treatments with N levels of 0.94, 1.69, 2.09, 3.04, 3.23, and 3.97% N, as fed, where Lys was limiting, with six replicates and one pig per unit. The challenge consisted of an initial LPS dose of 30 µg/kg BW via intramuscular (IM) injection and a subsequent dose of 33.6 µg/kg BW after 48 h. The experimental period lasted 11 d and was composed of a 7-d adaptation and a subsequent 4-d sampling period in which N intake (NI), N excretion (NEX), and N deposition (ND) were evaluated. Inflammatory mediators and rectal temperature were assessed during the 4-d collection period. A three-way interaction (N levels × LPS challenge × time, P < 0.05) for IgG was observed. Additionally, two-way interactions (challenge × time, P < 0.05) were verified for IgA, ceruloplasmin, transferrin, haptoglobin, α-1-acid glycoprotein, total protein, and rectal temperature; and (N levels × time, P < 0.05) for transferrin, albumin, haptoglobin, total protein, and rectal temperature. LPS-challenged pigs showed lower (P < 0.05) feed intake. A two-way interaction (N levels × LPS challenge, P < 0.05) was observed for NI, NEX, and ND, with a clear dose-response (P < 0.05). LPS-challenged pigs showed lower NI and ND at 2.09% N and 1.69 to 3.97% N (P < 0.05), respectively, and higher NEX at 3.23% N (P < 0.05). The parameters obtained by a nonlinear model (N maintenance requirement, NMR and theoretical maximum N deposition, NDmaxT) were 152.9 and 197.1 mg/BWkg0.75/d for NMR, and 3,524.7 and 2,077.8 mg/BWkg0.75/d for NDmaxT, for control and LPS-challenged pigs, respectively. The estimated digestible Lys requirements were 1,994.83 and 949.16 mg/BWkg0.75/d for control and LPS-challenged pigs, respectively. The daily digestible Lys intakes required to achieve 0.68 and 0.54 times the NRmaxT value were 18.12 and 8.62 g/d, respectively, and the optimal dietary digestible Lys concentration may change depending on the feed intake levels. Based on the derived model parameters obtained in the N balance trial with lower cost and time, it was possible to differentiate the digestible Lys requirement for swine under challenging conditions.

Effects of Ambient Temperature on the Performance and Thermoregulatory Responses of Commercial and Crossbred (Brazilian Piau Purebred Sires × Commercial Dams) Growing-Finishing Pigs.

The study aimed at evaluating the effects of high ambient temperature (HT: 30 °C) on the thermoregulatory responses and performance of commercial and Piau crossbred (Brazilian Piau breed sires × commercial genotype dams) growing pigs. Commercial and Piau crossbred pigs were reared under thermoneutral (TN: 22 °C) or HT conditions during a 14-day experimental period. Feeding (daily) and animals (beginning and end) were weighted to obtain performance parameters. Skin and rectal temperatures, respiratory rate, and blood parameters were also measured. At the end of the trial (day 15), the animal's backfat thickness (BF) and loin eye area (LEA) were measured. No interaction (p > 0.05) between the genetic group and ambient temperature was observed for any performance trait. Irrespective of ambient temperature, Piau crossbred pigs had a similar feed intake (ADFI, 2615 g/day, on average; p > 0.05), lower daily weight gain (ADG, -234 g/day; p < 0.01), and a higher feed conversion ratio (FCR, +0.675 g/g; p < 0.01). There was interaction (p = 0.01) between genotype and ambient temperature for the LEA that decreased significantly in response to HT in commercial pigs (-6.88 cm2) and did not differ in response to ambient temperature in Piau crossbred pigs (29.14 cm2, on average; p > 0.05). Piau crossbred pigs had greater BF (+7.2 mm; p < 0.01) than commercial pigs. Regardless of the genetic group, exposure of pigs to HT resulted in decreased ADFI (-372 g/day; p < 0.01), ADG (-185 g/day; p < 0.01), and a higher FCR (+0.48 g/g; p = 0.01). Ambient temperature did not affect lipid deposition. Pigs at HT had an increased respiratory rate (+38 bpm; p < 0.01) and a long-lasting increase in skin and rectal temperatures compared to TN pigs. Total concentrations of triiodothyronine (T3) and thyroxine (T4) were not affected by ambient temperature in commercial pigs, whereas Piau crossbred pigs kept at 30 °C had a transient decrease in both hormones at day 2 (p < 0.01). Serum cortisol concentrations were not affected (p > 0.05) by genotype nor ambient temperature. In summary, Piau crossbred pigs had lower efficiency using nutrients for growth in association with increased lipid deposition when compared to commercial pigs. In response to HT, commercial pigs had a decreased LEA, whereas no effect was observed for Piau crossbred pigs. Apart from that, commercial and Piau crossbred pigs had a similar magnitude of thermoregulatory responses activation in response to HT, evidencing their innate survival-oriented function.

O Programa Nacional de Controle do Tabagismo no município do Rio de Janeiro: ligações que promovem oportunidades / The National Smoking Control Program in Rio de Janeiro district: telephone calls that promotes opportunities

Resumo Introdução Apesar de o Programa Nacional de Controle do Tabagismo (PNCT) ser um dos mais efetivos da América Latina, resultando em contínua queda do hábito de fumar até 2013, sua prevalência vem se mostrando importante em algumas regiões do Brasil.Objetivo: Analisar a situação da rede do PNCT no município do Rio de Janeiro no ano de 2015 em relação à captação de tabagistas sob a óptica da análise de redes sociais. Método A pesquisa possui caráter exploratório-descritivo e qualitativo. As informações foram obtidas por meio de ligações telefônicas, realizadas a todas as unidades de saúde participantes do PNCT, totalizando 183 ligações. Para o complemento da análise à abordagem pela análise de redes sociais, foi utilizada a metodologia conhecida como matriz FOFA (Forças, Oportunidades, Fraquezas e Ameaças). Resultados Do total de 183 unidades, foi obtida resposta de 68. Apesar de mais da metade dos atendimentos ter ocorrido por encaminhamento dos profissionais de saúde, quantidade significativa se deu por meios informais. Conclusão Conclui-se que existem diversos fatores relacionados a forças, oportunidades, fraquezas e ameaças. Porém, se pouco falta em relação a recursos e estratégias para a melhoria da captação, muito há a fazer para que as ligações telefônicas sejam oportunidades de mudança.

ABSTRACT Background Although the National Smoking Control Program - PNCT is one of the most effective in Latin America, resulting in a continuous decline in the prevalence of smoking until 2013, its prevalence has been shown to be important in some regions of Brazil. Objective To analyze the situation of the PNCT network in the Municipality of Rio de Janeiro in 2015, regarding the uptake of smokers from the perspective of Social Network Analysis. Method This is an exploratory-descriptive and qualitative study. Information was obtained through telephone calls made to all Health Units participating in the PNCT, totaling 183 calls. To complement the analysis of the approach through the analysis of social networks, we used the methodology known as SWOT matrix - strengths, opportunities, weaknesses, and threats. Results From a total of 183 units, 68 responded. Although more than half of the consultations took place through referrals from health professionals, a significant number took place through informal means.Conclusion: It is concluded that several factors related to weaknesses, strengths, opportunities, and threats exist. However, if there is little lack of resources and strategies to improve uptake, there is much to be done so that telephone calls are opportunities for change.

Effects of heat stress on performance and thermoregulatory responses of Piau purebred growing pigs.

The study of how different breeds adapt to heat stress and the further understanding of mechanisms underlying pigs thermotolerance is of utmost importance to attenuate the negative effects of heat stress on pigs welfare, physiology, and performance. Therefore, this study aimed at evaluating the effects of ambient temperature on performance and thermoregulatory responses of Piau purebred pigs. The Brazilian native pig breed Piau is a fat-type breed characterized by adaptability and resistance to diseases. To achieve our goal, Piau purebred pigs (65 kg initial BW) were allocated to one of the two constant ambient temperature conditions: thermoneutral (22 °C; n = 11) and heat stress (30 °C; n = 11). The experimental period lasted 15 days (days 1 to 15). Pigs were individually weighed at the beginning and end of the experimental period. Body and rectal temperatures, respiratory rate and blood indicators of stress and metabolism were measured throughout the experiment. Piau purebred pigs exposed to 30 °C had lower (p < 0.05) feed intake, body weight gain and final body weight than pigs at 22 °C. Feed conversion rate was not affected (p > 0.05) by ambient temperature. Irrespective of ambient temperature, pigs had similar (p > 0.05) backfat thickness and loin eye area. Piau pigs at 30 °C had increased (p < 0.05) nape, dorsal, flank, and rectal temperatures and increased respiratory rate than their counterparts reared at 22 °C. In summary, our results show that Piau purebred pigs acclimation to 30 °C of ambient temperature is characterized by increased body skin temperature to optimize sensible heat loss to the environment; increased respiratory rate to promote latent heat loss; and by a concomitant reduced voluntary feed intake to reduce heat production associated with digestion and metabolic processes with negative effects on body weight gain.

Robustness and predictability of evolution in bottlenecked populations.

Deterministic and stochastic evolutionary processes drive adaptation in natural populations. The strength of each component process is determined by the population size: deterministic components prevail in very large populations, while stochastic components are the driving mechanisms in small ones. Many natural populations, however, experience intermittent periods of growth, moving through states in which either stochastic or deterministic processes prevail. This growth is often countered by population bottlenecks, which abound in both natural and laboratory populations. Here we investigate how population bottlenecks shape the process of adaptation. We demonstrate that adaptive trajectories in populations experiencing regular bottlenecks can be naturally scaled in time units of generations; with this scaling the time courses of adaptation, fitness variance, and genetic diversity all become relatively insensitive to the timing of population bottlenecks, provided the bottleneck size exceeds a few thousand individuals. We also include analyses at the genotype level to investigate the impact of population bottlenecks on the predictability and distribution of evolutionary pathways. Irrespective of the timing of population bottlenecks, we find that predictability increases with population size. We also find that predictability of the adaptive pathways increases in increasingly rugged fitness landscapes. Overall, our work reveals that both the adaptation rate and the predictability of evolutionary trajectories are relatively robust to population bottlenecks.

Selection for feed efficiency elicits different postprandial plasma metabolite profiles in response to poor hygiene of housing conditions in growing pigs.

This study was conducted to compare postprandial plasma concentrations of insulin, energy-related metabolites, and amino acids measured after a 6-week challenge consisting of exposure to good or poor hygiene of housing conditions of 24 growing pigs divergently selected for low-RFI (LRFI) and high-RFI (HRFI). Blood indicators of immune responses were assessed from samples collected before 0 (W0), and 3 (W3), and 6 weeks (W6) after pigs transfer to their respective hygiene of housing conditions. Plasma haptoglobin concentrations and blood neutrophil granulocyte numbers were greater in poor than in good hygiene of housing conditions at W3. Plasma concentrations of total immunoglobulin G were greater (p = 0.04) in poor than in good hygiene of housing conditions at W6. At W6, pigs were fitted with an intravenous catheter for serial blood samplings. Low-RFI pigs had greater insulin (p < 0.001) and lower triglyceride (p = 0.04) average plasma concentrations than HRFI pigs in both conditions. In poor hygiene of housing conditions, the peaks of insulin and glucose were observed earlier and that of insulin was greater in LRFI than in HRFI pigs. Irrespective of genetic line, average plasma concentrations of histidine, isoleucine, leucine, methionine, threonine, valine, and alanine were greater in poor compared with good hygiene of housing conditions. Only HRFI pigs had greater lysine, asparagine, proline, and tyrosine plasma concentrations in poor than in good hygiene of housing conditions. Conversely, arginine, tryptophan, proline, and tyrosine plasma concentrations were lower only for LRFI pigs housed in poor hygiene conditions. Our results suggest that, contrary to HRFI, LRFI pigs increase or maintain their utilization of tryptophan, arginine, and lysine when housed in poor hygiene conditions. This indicates that this difference may contribute to the better capacity of LRFI to cope with poor hygiene of housing conditions.

Influence of different photoinitiators on the resistance of union in bovine dentin: Experimental and microscopic study.

BACKGROUND: To evaluate in vitro the influence of photoinitiators on the microtensile strength of adhesive systems and composite resins in bovine dentin. MATERIAL AND METHODS: Forty dentin obtained from bovine teeth were randomly distributed in four groups (n = 10) according to the different adhesive systems and composite resins used: G1 - AAPS + VAPS (Ambar APS + Vittra APS); G2- AAPS + O (Ambar APS + Opallis); G3 - A + VAPS (Ambar + Vittra APS) and G4 - A + O (Ambar + Opallis). After restoration with the composite, the samples were sectioned to obtain toothpicks that were subjected to the microtensile and nanofiltration test (1.0 mm/min). RESULTS: The Kruskal-Wallis test did not show significant differences between the groups (p<0.05). The values in MPa were: AAPS + VAPS - 19.56 MPa; AAPS + O - 19.77 MPa; A + VAPS - 17.78 MPa; A + O - 22.44 MPa. The result of the Mann-Whitney test showed no significant differences depending on the adhesive (Ambar Universal- 19.11 MPa, Ambar APS Universal- 21.70 MPa) and the composite resin used (Vittra APS- 18.75 MPa, Opallis - 23.75 MPa). The AAPS + VAPS and AAPS + O groups showed intense silver nitrate infiltration. The A + APS group showed a moderate infiltration and the A + O group had a mild infiltration in the adhesive system/dentin interface. CONCLUSIONS: The use of different photoinitiators in the composition of adhesive systems and restorative composites did not affect their bond strength values and the presence of water in the solvent of the APS photoinitiator system had a negative influence, increasing the degree of infiltration in the hybrid layer when compared to the camphorquinone photoinitiator. Key words:Dentin, adhesive systems, composite resin, photoinitiators, tensile strength, nano-infiltration.